Cellular and Molecular Biology

Scholar Year: 2018/2019

Courses

Head Teacher Responsability Maria Helena de Figueiredo Ramos Caria Head

Weekly workload Hours/week T TP P PL TC S E EL OT TPL O OT/PL Type of classes 4 Lectures Type Teacher Classes Hours Theoretical Totals 1 4,00 Helena Caria   4,00

Teaching language

Portuguese

Intended learning outcomes (Knowledges, skills and competencies to be developed by the students)

The aim of the course is to enable students to acquire skills in cell and molecular biology that allow them
to act as health pofessionals in the development of support structures for research and clinical testing,
processing and interpretation of biological signals in the area of cellular and molecular biology.
It is intended that at the end of this course the student is able to:
• Understanding the cell division process, replication, transcription and translation of nucleic acids, with
particular focus on the failures of vital physiological processes;
• Knew new cell biology and molecular technologies applied in different areas, particularly in the areas of
health.

Syllabus

Cell Biology Module
1. Cellular and Molecular Biology and Biomedical Engineering. Perspectives in the XXI century.
2. Cell hierarchy.
3 .Cellular structures: prions, viruses, prokaryotes and eukaryotes
4 . Cell membrane.
5. Matrix organelles.
6. Proteins and membrane transport systems
7 . Cellular compartments and molecular synchrony
8. Nucleic acids. Regulation of gene transcription. The healing process. Apoptosis
9. Cell cycle: regulation and concept of tumor
10. Prokaryotes cells
11 . Notions of phage therapy
Molecular Biology Module
1. Central Dogma of Molecular Biology and prions. The non- replicating protein
2 . Processing of proteins as a means of regulating gene
3 . Methylation and physiological processes such as phosphorylation homeostasis
4 . Mutations
6 . Restriction enzymes
7. Eletrophoresys
9.PCR and cloning
10.DNA Sequencing
11. Biotechnological applications with plasmids

Demonstration of the syllabus coherence with the UC intended learning outcomes

The syllabus set achieves these objectives, in that it contains a set of topics essential to the learning
expected. Works with the student basic concepts for understanding and knowledge of the diversity of
cellular and molecular processes in living beings as well as for the field of vital physiological mechanisms,
such as cell division and specialization in this molecular process that enable you to acquire knowledge
allowing interventions in the fields of health.

Teaching methodologies

Expositive, demonstrative, participative and problem solving. The unit can be effected by: (a) continuous
evaluation, ongoing at the school and that results in the exemption examination period, if the final grade is
equal to or higher than 10, and assuming the formative and summative dimensions; (b) exam, which takes
the summative dimension. The final classification (FC) of curricular unit for continuous evaluation is
obtained from the marks obtained in the written component (T).
The written component consists of two written tests (T1 and T2). The minimum grade in each test is 9.5
values. The final grade is calculated from the following formula: CF = 0.50*T1 + 0.50*T2. The classification of all assessment instruments is expressed on a scale of 0 to 20, and the weighted value of the marks
obtained must be equal or greater than 10. The exam consists in a written test, in which the approval will
require a rating equal to or higher than 10.

Demonstration of the teaching methodologies coherence with the curricular unit's intended learning outcomes

In this curricular unit, the syllabus is organized into two semi-autonomous modules, which are related to
the learning methodologies and with the organization of semester activities. There are flexibility in teaching
practices and organization, allowing a more effective learning process of students since they are focus on
the learning process, assuming the management for the development of their knowledge and skills.
Moreover, the method implies that expository-participatory students carry out previously some research
for better discuss topics. Thus, they should become proactive and not just knowing the contents, but also
to be able to discuss the contents and even be able to propose developments of new laboratory
procedures of molecular tests.

Bibliografia

Avila, V. (1995). Biology –Investigating Life on Earth. 2ªed. Boston: Jones and Bartlet Publishers.
Azevedo, C.(2001). Biologia Celular e Molecular. 3ªEd. Lisboa: Lidel. 1999. ISBN 972-757-100-X.
Baynes,S. Bioquímica Médica. Lisboa: Lusodidacta.
Lozano, JA et al. (2003).Bioquímica Y Biologia Molecular para ciencias de la salud. Madrid: Interamericana
McGraw-Hill. 2ªed.
Lodish, H et al. (1995). Molecular Cell Biology. 3ªed. New York: Scientific American Books.
McKee, T.; McKee, J.(2003). Biochemistry: the molecular basis of life. 3ªed. New York: McGraw-Hill.
Quintas, A., Ponces Freire, A.; Halpern, M. (2008). Bioquimica: Organização molecular da vida. 1ª Ed.
Lisboa: Lidel. ISBN: 978-972-757-431-5
Stansfield, W; Colomé, J, Cano. (1998). Biologia Molecular e Celular – Teoria e Exercícios. Trad.
Portuguesa. Lisboa:McGraw-Hill